Ontology highlight
ABSTRACT:
SUBMITTER: Giacca M
PROVIDER: S-EPMC44350 | biostudies-other | 1994 Jul
REPOSITORIES: biostudies-other
Giacca M M Zentilin L L Norio P P Diviacco S S Dimitrova D D Contreas G G Biamonti G G Perini G G Weighardt F F Riva S S
Proceedings of the National Academy of Sciences of the United States of America 19940701 15
A highly sensitive procedure was developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells. The method, which does not require cell synchronization or permeabilization, entails the absolute quantification, by a competitive PCR procedure in newly synthesized DNA samples, of the abundance of neighboring DNA fragments distributed along a given genomic region. This procedure was utilized for mapping the start site of DNA replication in ...[more]