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An ACAP1-containing clathrin coat complex for endocytic recycling.

ABSTRACT: Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings, stimulation-dependent recycling of integrin that is critical for cell migration and insulin-stimulated recycling of glucose transporter type 4 (Glut4), which is required for glucose homeostasis. These findings not only advance a basic understanding of an early mechanistic step in endocytic recycling but also shed key mechanistic insights into major physiological events for which this transport plays a critical role.

SUBMITTER: Li J

PROVIDER: S-EPMC2064835 | biostudies-other | 2007 Jul

REPOSITORIES: biostudies-other

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An ACAP1-containing clathrin coat complex for endocytic recycling.

Li Jian J Peters Peter J PJ Bai Ming M Dai Jun J Bos Erik E Kirchhausen Tomas T Kandror Konstantin V KV Hsu Victor W VW

The Journal of cell biology 20070701 3

Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings, stimulation-dependent recycling of integrin that is critical for cell migration and insulin-stimulated recycling of glucose transporter type 4 (Glut4), which is required for glucose homeostasis. The ...[more]

PMID: 17664335

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