Ontology highlight
ABSTRACT:
SUBMITTER: Takiff HE
PROVIDER: S-EPMC206550 | biostudies-other | 1992 Mar
REPOSITORIES: biostudies-other
Takiff H E HE Baker T T Copeland T T Chen S M SM Court D L DL
Journal of bacteriology 19920301 5
The mini-Tn10 transposon (delta 16 delta 17Tn10) confers tetracycline resistance. When inserted between a gene and its promoter, it blocks transcription and prevents expression of that gene. Tetracycline in the medium induces divergent transcription of the tetA and tetR genes within the transposon, and this transcription extends beyond the transposon in both directions into the bacterial genes. If the mini-Tn10 inserts between an essential bacterial gene and its promoter, the insertion mutation ...[more]