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Characterization of the defect in the Escherichia coli mutT1 mutator gene.


ABSTRACT: With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.

SUBMITTER: Bhatnagar SK 

PROVIDER: S-EPMC208933 | biostudies-other | 1990 May

REPOSITORIES: biostudies-other

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Characterization of the defect in the Escherichia coli mutT1 mutator gene.

Bhatnagar S K SK   Bullions L C LC   Lew G G   Bessman M J MJ  

Journal of bacteriology 19900501 5


With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene. ...[more]

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