Ontology highlight
ABSTRACT:
SUBMITTER: Schmid B
PROVIDER: S-EPMC2144057 | biostudies-other | 1998 Jul
REPOSITORIES: biostudies-other
Protein science : a publication of the Protein Society 19980701 7
The general diffusion porin from Rhodopseudomonas blastica was produced in large amounts in Escherichia coli inclusion bodies and (re)natured to the exact native structure. Here, we report on 13 mutants at the pore eyelet giving rise to new diffusion properties as measured in planar lipid bilayer experiments. The crystal structures of seven of these mutants were established. The effects of charge-modifying mutations at the pore eyelet are consistent with the known selectivity for cations. Deleti ...[more]