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Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P.


ABSTRACT: The 2.1 A resolution crystal structure of flavin reductase P with the inhibitor nicotinamide adenine dinucleotide (NAD) bound in the active site has been determined. NAD adopts a novel, folded conformation in which the nicotinamide and adenine rings stack in parallel with an inter-ring distance of 3.6 A. The pyrophosphate binds next to the flavin cofactor isoalloxazine, while the stacked nicotinamide/adenine moiety faces away from the flavin. The observed NAD conformation is quite different from the extended conformations observed in other enzyme/NAD(P) structures; however, it resembles the conformation proposed for NAD in solution. The flavin reductase P/NAD structure provides new information about the conformational diversity of NAD, which is important for understanding catalysis. This structure offers the first crystallographic evidence of a folded NAD with ring stacking, and it is the first enzyme structure containing an FMN cofactor interacting with NAD(P). Analysis of the structure suggests a possible dynamic mechanism underlying NADPH substrate specificity and product release that involves unfolding and folding of NADP(H).

SUBMITTER: Tanner JJ 

PROVIDER: S-EPMC2144397 | biostudies-other | 1999 Sep

REPOSITORIES: biostudies-other

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Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P.

Tanner J J JJ   Tu S C SC   Barbour L J LJ   Barnes C L CL   Krause K L KL  

Protein science : a publication of the Protein Society 19990901 9


The 2.1 A resolution crystal structure of flavin reductase P with the inhibitor nicotinamide adenine dinucleotide (NAD) bound in the active site has been determined. NAD adopts a novel, folded conformation in which the nicotinamide and adenine rings stack in parallel with an inter-ring distance of 3.6 A. The pyrophosphate binds next to the flavin cofactor isoalloxazine, while the stacked nicotinamide/adenine moiety faces away from the flavin. The observed NAD conformation is quite different from  ...[more]

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2022-07-31 | GSE180956 | GEO