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Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.

ABSTRACT: For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.

SUBMITTER: Hashimoto Y

PROVIDER: S-EPMC228036 | biostudies-other | 1995 Mar

REPOSITORIES: biostudies-other

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Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.

Hashimoto Y Y Itho Y Y Fujinaga Y Y Khan A Q AQ Sultana F F Miyake M M Hirose K K Yamamoto H H Ezaki T T

Journal of clinical microbiology 19950301 3

For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level. ...[more]

PMID: 7751397

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