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Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene.

ABSTRACT: Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences.

SUBMITTER: Battles JK

PROVIDER: S-EPMC228160 | biostudies-other | 1995 May

REPOSITORIES: biostudies-other

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Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene.

Battles J K JK Williamson J C JC Pike K M KM Gorelick P L PL Ward J M JM Gonda M A MA

Journal of clinical microbiology 19950501 5

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences. ...[more]

PMID: 7542270

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