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Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.

ABSTRACT: A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.

SUBMITTER: Respess RA

PROVIDER: S-EPMC232750 | biostudies-other | 1997 May

REPOSITORIES: biostudies-other

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Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.

Respess R A RA Butcher A A Wang H H Chaowanachan T T Young N N Shaffer N N Mastro T D TD Biryahwaho B B Downing R R Tanuri A A Schechter M M Pascu R R Zekeng L L Kaptué L L Gürtler L L Eberle J J Ellenberger D D Fridlund C C Rayfield M M Kwok S S

Journal of clinical microbiology 19970501 5

A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of ...[more]

PMID: 9114428

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