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Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines.

ABSTRACT: Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed.

SUBMITTER: van Schothorst EM

PROVIDER: S-EPMC2363097 | biostudies-other | 1999 Jul

REPOSITORIES: biostudies-other

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Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines.

van Schothorst E M EM Mohkamsing S S van Gurp R J RJ Oosterhuis J W JW van der Saag P T PT Looijenga L H LH

British journal of cancer 19990701 10

Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivatio ...[more]

PMID: 10408400

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