Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
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ABSTRACT:
SUBMITTER: Tanji Y
PROVIDER: S-EPMC237315 | biostudies-other | 1994 Dec
REPOSITORIES: biostudies-other
Publications
Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
Tanji Y Y Hijikata M M Hirowatari Y Y Shimotohno K K
Journal of virology 19941201 12
Hepatitis C virus (HCV) serine proteinase (Cpro-2) is responsible for the processing of HCV nonstructural (NS) protein processing. To clarify the mechanism of Cpro-2-dependent processing, pulse-chase and mutation analyses were performed by using a transient protein production system in cultured cells. Pulse-chase study revealed the sequential production of HCV-NS proteins. Production of p70(NS3) and p66(NS5B) were rapid. An 89-kDa processing intermediate protein (p89) was observed during the ear ...[more]
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