15N metabolic labeling of mammalian tissue with slow protein turnover.
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ABSTRACT:
SUBMITTER: McClatchy DB
PROVIDER: S-EPMC2527585 | biostudies-other | 2007 May
REPOSITORIES: biostudies-other
Publications
15N metabolic labeling of mammalian tissue with slow protein turnover.
McClatchy Daniel B DB Dong Meng-Qiu MQ Wu Christine C CC Venable John D JD Yates John R JR
Journal of proteome research 20070322 5
We previously reported the metabolic 15N labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% 15N enrichment throughout all tissues of the rat. A high 15N enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of disease targeting any tissue. ...[more]
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