Ontology highlight
ABSTRACT:
SUBMITTER: Zhou Z
PROVIDER: S-EPMC2567456 | biostudies-other | 2008 Sep
REPOSITORIES: biostudies-other
Zhou Zhemin Z Hashimoto Yoshiteru Y Shiraki Kentaro K Kobayashi Michihiko M
Proceedings of the National Academy of Sciences of the United States of America 20080922 39
Several general mechanisms of metallocenter biosynthesis have been reported and reviewed, and in all cases, the components or subunits of an apoprotein remain in the final holoprotein. Here, we first discovered that one subunit of an apoenzyme did not remain in the functional holoenzyme. The cobalt-containing low-molecular-mass nitrile hydratase (L-NHase) of Rhodococcus rhodochrous J1 consists of beta- and alpha-subunits encoded by the nhlBA genes, respectively. An ORF, nhlE, just downstream of ...[more]