Ontology highlight
ABSTRACT:
SUBMITTER: Willoughby JJ
PROVIDER: S-EPMC258032 | biostudies-other | 1991 Jul
REPOSITORIES: biostudies-other
Willoughby J J JJ Russell W C WC Thirkell D D Burdon M G MG
Infection and immunity 19910701 7
Urease from ureaplasmas was purified by immunoaffinity chromatography, and the N-terminal amino acid sequence was determined for two of the three subunits. These sequences were used to design primers for a polymerase chain reaction (PCR) that amplified most of the gene coding for one of the subunits. By using a novel "PCR walking" technique, we synthesized almost the complete locus on two overlapping PCR products. We present here a partial nucleotide sequence of the urease locus from Ureaplasma ...[more]