Ontology highlight
ABSTRACT:
SUBMITTER: Segers RP
PROVIDER: S-EPMC258794 | biostudies-other | 1990 Jul
REPOSITORIES: biostudies-other
Segers R P RP van der Drift A A de Nijs A A Corcione P P van der Zeijst B A BA Gaastra W W
Infection and immunity 19900701 7
A thermolabile hemolysin from Leptospira interrogans serovar hardjo, strain Sponselee, was shown to specifically degrade sphingomyelin. Nucleotide sequence determination revealed that sphingomyelinase activity was encoded by an open reading frame of 1,668 nucleotides. Although a putative signal sequence could be identified, no evidence for protein export in either L. interrogans or Escherichia coli was obtained. The apparent molecular mass of the expression product in E. coli minicells was 41.2 ...[more]