Ontology highlight
ABSTRACT:
SUBMITTER: Lo RY
PROVIDER: S-EPMC258898 | biostudies-other | 1991 Oct
REPOSITORIES: biostudies-other
Lo R Y RY Strathdee C A CA Shewen P E PE Cooney B J BJ
Infection and immunity 19911001 10
A serotype-specific antigen of Pasteurella haemolytica A1 encoded on the recombinant plasmid pSSA1 is characterized. Nucleotide sequence analysis of the insert DNA in pSSA1 identified the gene ssaI, which codes for a protein of approximately 100 kDa. In vivo labeling of pSSA1-encoded protein in Escherichia coli maxicells showed the expression of a 100-kDa protein from the insert DNA on the recombinant plasmid. Northern blot and primer extension analyses were used to identify the mRNA transcript ...[more]