Ontology highlight
ABSTRACT:
SUBMITTER: Unal S
PROVIDER: S-EPMC265364 | biostudies-other | 1992 Jul
REPOSITORIES: biostudies-other
Unal S S Hoskins J J Flokowitsch J E JE Wu C Y CY Preston D A DA Skatrud P L PL
Journal of clinical microbiology 19920701 7
A polymerase chain reaction (PCR)-based test was developed for the detection of mecA in staphylococci. To facilitate this process, a rapid cell lysis procedure was established for the release of DNA from staphylococcal strains. Primers based on the DNA sequence of the mecA gene from Staphylococcus aureus were used in PCRs to screen for the presence of this gene in a total of 98 staphylococcal isolates. Fifty-one isolates were mecA positive (17 S. aureus strains and 34 coagulase-negative staphylo ...[more]