Ontology highlight
ABSTRACT:
SUBMITTER: Altamirano M
PROVIDER: S-EPMC265465 | biostudies-other | 1992 Aug
REPOSITORIES: biostudies-other
Altamirano M M Kelly M T MT Wong A A Bessuille E T ET Black W A WA Smith J A JA
Journal of clinical microbiology 19920801 8
We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 spu ...[more]