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Laser-guidance based detection of cells with single-gene modification.


ABSTRACT: An optical method with single-gene sensitivity for cell detection based on laser guidance was explored. Guided by the optical force from a weakly focused laser beam, a cell will move along the laser axis. Cells with different properties experience different optical forces and thus guidance speeds. The guidance speeds of the TC-1 cell and its genetically modified counterpart with only one gene change, L-10 cell, were studied under the same conditions. The results demonstrated that this laser guidance-based speed-measurement method can precisely distinguish cells that differ by only one gene.

SUBMITTER: Ma Z 

PROVIDER: S-EPMC2682740 | biostudies-other | 2008 May

REPOSITORIES: biostudies-other

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