Ontology highlight
ABSTRACT:
SUBMITTER: Hayashi-Takanaka Y
PROVIDER: S-EPMC3159477 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Hayashi-Takanaka Yoko Y Yamagata Kazuo K Wakayama Teruhiko T Stasevich Timothy J TJ Kainuma Takashi T Tsurimoto Toshiki T Tachibana Makoto M Shinkai Yoichi Y Kurumizaka Hitoshi H Nozaki Naohito N Kimura Hiroshi H
Nucleic acids research 20110516 15
Histone modifications play an important role in epigenetic gene regulation and genome integrity. It remains largely unknown, however, how these modifications dynamically change in individual cells. By using fluorescently labeled specific antigen binding fragments (Fabs), we have developed a general method to monitor the distribution and global level of endogenous histone H3 lysine modifications in living cells without disturbing cell growth and embryo development. Fabs produce distinct nuclear p ...[more]