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HCO 3--dependent volume regulation in alpha-cells of the rat endocrine pancreas.


ABSTRACT: Ion transport activity in pancreatic alpha-cells was assessed by studying cell volume regulation in response to anisotonic solutions. Cell volume was measured by a video imaging method, and cells were superfused with either 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid-buffered or HCO(3)(-) -buffered solutions. alpha-Cells did not exhibit a regulatory volume increase (RVI) in response to cell shrinkage caused by hypertonic solutions. A RVI was observed, however, in cells that had first undergone a regulatory volume decrease (RVD), but only in HCO(3)(-)-buffered solutions. RVI was also observed in response to a HCO(3)(-) -buffered hypertonic solution in which the glucose concentration was increased from 4 to 20 mM. The post-RVD RVI and the glucose-induced RVI were both inhibited by 10 microM 5-(N-methyl-N-isobutyl) amiloride or 100 microM 2,2'-(1,2-ethenediyl) bis (5-isothio-cyanatobenzenesulfonic acid), but not by 10 microM benzamil nor 10 microM bumetanide. These data suggest that Na(+)-H(+) exchangers and Cl(-)-HCO(3)(-) exchangers contribute to volume regulation in alpha-cells.

SUBMITTER: Davies SL 

PROVIDER: S-EPMC2691524 | biostudies-other | 2009 Jul

REPOSITORIES: biostudies-other

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