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Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis.

ABSTRACT: A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-sensitive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.

SUBMITTER: Hwang SY

PROVIDER: S-EPMC2717493 | biostudies-other | 2003 Jun

REPOSITORIES: biostudies-other

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Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis.

Hwang Seung-Young SY Han Hye-Jin HJ Kim So-Hee SH Park Sae-Gwang SG Seog Dae-Hyun DH Kim Na Ri NR Han Jin J Chung Joon-Yong JY Kho Weon-Gyu WG

The Korean journal of parasitology 20030601 2

A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-sensitive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino a ...[more]

PMID: 12815327

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