Ontology highlight
ABSTRACT:
SUBMITTER: Tabata KV
PROVIDER: S-EPMC2776102 | biostudies-other | 2009 Nov
REPOSITORIES: biostudies-other
Tabata Kazuhito V KV Sato Ken K Ide Toru T Nishizaka Takayuki T Nakano Akihiko A Noji Hiroyuki H
The EMBO journal 20090917 21
Selective protein export from the endoplasmic reticulum is mediated by COPII vesicles. Here, we investigated the dynamics of fluorescently labelled cargo and non-cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. Single-molecule analysis showed that the Sar1p-Sec23/24p-cargo complex, but not the Sar1p-Sec23/24p complex, undergoes partial dimerization before Sec13/31p recruitment. On addition of a complete COPII mixture ...[more]