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Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities.

ABSTRACT: mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

SUBMITTER: Schloss PD

PROVIDER: S-EPMC2786419 | biostudies-other | 2009 Dec

REPOSITORIES: biostudies-other

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Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities.

Schloss Patrick D PD Westcott Sarah L SL Ryabin Thomas T Hall Justine R JR Hartmann Martin M Hollister Emily B EB Lesniewski Ryan A RA Oakley Brian B BB Parks Donovan H DH Robinson Courtney J CJ Sahl Jason W JW Stres Blaz B Thallinger Gerhard G GG Van Horn David J DJ Weber Carolyn F CF

Applied and environmental microbiology 20091002 23

mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing ...[more]

PMID: 19801464

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