Ontology highlight
ABSTRACT:
SUBMITTER: Oke OA
PROVIDER: S-EPMC2823865 | biostudies-other | 2010 Feb
REPOSITORIES: biostudies-other
Oke Olusola A OA Burgess Stan A SA Forgacs Eva E Knight Peter J PJ Sakamoto Takeshi T Sellers James R JR White Howard H Trinick John J
Proceedings of the National Academy of Sciences of the United States of America 20100125 6
Using electron microscopy and image processing, we have observed myosin 5a modified with lever arms of different lengths (four, six, and eight calmodulin-binding IQ domains) and orientations walking along actin filaments. Step lengths were dependent on lever length: 8IQ > 6IQ > 4IQ, which is consistent with myosin 5a having evolved to walk straight along actin. Lead heads were mostly in the prepowerstroke state, tethered there by the trail head. However, improved image processing showed that in ...[more]