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Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression.


ABSTRACT: MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored.Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter.We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1beta stimulation.

SUBMITTER: Kasza A 

PROVIDER: S-EPMC2829564 | biostudies-other | 2010 Feb

REPOSITORIES: biostudies-other

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Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression.

Kasza Aneta A   Wyrzykowska Paulina P   Horwacik Irena I   Tymoszuk Piotr P   Mizgalska Danuta D   Palmer Karren K   Rokita Hanna H   Sharrocks Andrew D AD   Jura Jolanta J  

BMC molecular biology 20100206


<h4>Background</h4>MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored.<h4>Results</h4>Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription facto  ...[more]

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