Browse
Submit Data
Databases
API
Help

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ultrasensitive electrochemical immunosensor for oral cancer biomarker IL-6 using carbon nanotube forest electrodes and multilabel amplification.

ABSTRACT: Squamous cell carcinomas of head and neck (HNSCC) are associated with immune, inflammatory, and angiogenic responses involving interleukin-6 (IL-6). This article reports an ultrasensitive electrochemical immunosensor for human IL-6 and proof-of-concept studies of IL-6 detection in HNSCC cells. Single wall carbon nanotube (SWNT) forests with attached capture antibodies (Ab(1)) for IL-6 were used in an electrochemical sandwich immunoassay protocol using enzyme label horseradish peroxidase (HRP) to measure very low (

SUBMITTER: Malhotra R

PROVIDER: S-EPMC2854872 | biostudies-other | 2010 Apr

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Publications

Ultrasensitive electrochemical immunosensor for oral cancer biomarker IL-6 using carbon nanotube forest electrodes and multilabel amplification.

Malhotra Ruchika R Patel Vyomesh V Vaqué Jose Pedro JP Gutkind J Silvio JS Rusling James F JF

Analytical chemistry 20100401 8

Squamous cell carcinomas of head and neck (HNSCC) are associated with immune, inflammatory, and angiogenic responses involving interleukin-6 (IL-6). This article reports an ultrasensitive electrochemical immunosensor for human IL-6 and proof-of-concept studies of IL-6 detection in HNSCC cells. Single wall carbon nanotube (SWNT) forests with attached capture antibodies (Ab(1)) for IL-6 were used in an electrochemical sandwich immunoassay protocol using enzyme label horseradish peroxidase (HRP) to ...[more]

PMID: 20192182

Similar Datasets

Ultrasensitive immunosensor for cancer biomarker proteins using gold nanoparticle film electrodes and multienzyme-particle amplification.

Project description:A densely packed gold nanoparticle platform combined with a multiple-enzyme labeled detection antibody-magnetic bead bioconjugate was used as the basis for an ultrasensitive electrochemical immunosensor to detect cancer biomarkers in serum. Sensitivity was greatly amplified by synthesizing magnetic bioconjugates particles containing 7500 horseradish peroxidase (HRP) labels along with detection antibodies (Ab2) attached to activated carboxyl groups on 1 microm diameter magnetic beads. These sensors had sensitivity of 31.5 microA mL ng(-1) and detection limit (DL) of 0.5 pg mL(-1) for prostate specific antigen (PSA) in 10 microL of undiluted serum. This represents an ultralow mass DL of 5 fg PSA, 8-fold better than a previously reported carbon nanotube (CNT) forest immunosensor featuring multiple labels on carbon nanotubes, and near or below the normal serum levels of most cancer biomarkers. Measurements of PSA in cell lysates and human serum of cancer patients gave excellent correlations with standard ELISA assays. These easily fabricated AuNP immunosensors show excellent promise for future fabrication of bioelectronic arrays.

| S-EPMC2666939 | biostudies-literature

An ultrasensitive enzyme-free electrochemical immunosensor based on redox cycling amplification using methylene blue.

Project description:We report a new enzyme-free electrochemical sensor for ultrasensitive measurements of protein biomarkers in plasma and whole blood samples based on a unique electrochemical-chemical-chemical (ECC) redox cycling signal amplification scheme. This scheme uses methylene blue (MB) as a redox indicator which undergoes an endergonic reaction with Ru(NH3)63+ and a highly exergonic reaction with tris(2-carboxyethyl)phosphine (TCEP). This approach offers improved detection sensitivity and sensor stability compared with enzyme-based ECC redox cycling techniques, while involving a simpler sensor modification process and detection protocol. This redox cycling scheme was combined with a robust immunosandwich assay for quantitative measurements of protein biomarkers. For proof of principle, Plasmodium falciparum histidine-rich protein 2 (PfHRP2) was measured in human plasma and whole blood samples, which could be detected down to 10 fg mL-1 and 18 fg mL-1, respectively. Furthermore, this immunosensor exhibits high selectivity, excellent reproducibility and good stability for up to 2 weeks, making it a promising platform for point-of-care testing, especially for detecting extremely low biomarker concentrations in raw biofluids.

| S-EPMC5600201 | biostudies-literature

Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA.

Project description:A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This immunosensor was developed by using the trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) nanosheets (NiAuPt-NGs) as excellent labels and ?-cyclodextrin functionalized reduced graphene oxide nanosheets (CD-NGs) as the platform. The CD-NGs with high specific surface area good biocompatibility and the ideal dispersibility was used to capture the primary antibodies (Ab1) efficiently. The trimetallic NiAuPt-NGs nanocomposites were used as the labels for signal amplification, showing better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2), which is much better than that the monometallic Pt-NGs, bimetallic NiPt-NGs and AuPt-NGs due to the synergetic effect presented in NiAuPt-NGs. The NiAuPt-NGs nanocomposites consist of tightly coupled nanostructures of Au, Ni and Pt, which have neither an alloy nor a core-shell structure. Under the optimal conditions, a linear range from 0.001-100?ng/mL and a low detection limit of 0.27?pg/mL were obtained for CEA. The proposed electrochemical sandwich-type immunosensor may have a promising application in bioassay and it enriches the electrochemical immunoassays.

| S-EPMC4973229 | biostudies-literature

Ratiometric ultrasensitive electrochemical immunosensor based on redox substrate and immunoprobe.

Project description:In this work, we presented a ratiometric electrochemical immunosensor based on redox substrate and immunoprobe. Carboxymethyl cellulose-Au-Pb2+ (CMC-Au-Pb2+) and carbon-Au-Cu2+ (C-Au-Cu2+) nanocomposites were firstly synthesized and implemented as redox substrate and immunoprobe with strong current signals at -0.45?V and 0.15?V, respectively. Human immunoglobulin G (IgG) was used as a model analyte to examine the analytical performance of the proposed method. The current signals of CMC-Au-Pb2+ (Isubstrate) and C-Au-Cu2+ (Iprobe) were monitored. The effect of redox substrate and immunoprobe behaved as a better linear relationship between Iprobe/Isubstrate and Lg CIgG (ng mL-1). By measuring the signal ratio Iprobe/Isubstrate, the sandwich immunosensor for IgG exhibited a wide linear range from 1?fg mL-1 to 100?ng mL-1, which was two orders of magnitude higher than other previous works. The limit of detection reached 0.26?fg mL-1. Furthermore, for human serum samples, the results from this method were consistent with those of the enzyme linked immunosorbent assay (ELISA), demonstrating that the proposed immunoassay was of great potential in clinical diagnosis.

| S-EPMC5064308 | biostudies-literature

An ultrasensitive label-free electrochemical immunosensor based on signal amplification strategy of multifunctional magnetic graphene loaded with cadmium ions.

Project description:Herein, a novel and ultrasensitive label-free electrochemical immunosensor was proposed for quantitative detection of human Immunoglobulin G (IgG). The amino functionalized magnetic graphenes nanocomposites (NH2-GS-Fe3O4) were prepared to bond gold and silver core-shell nanoparticles (Au@Ag NPs) by constructing stable Au-N and Ag-N bond between Au@Ag NPs and -NH2. Subsequently, the Au@Ag/GS-Fe3O4 was applied to absorb cadmium ion (Cd(2+)) due to the large surface area, high conductivity and exceptional adsorption capability. The functional nanocomposites of gold and silver core-shell magnetic graphene loaded with cadmium ion (Au@Ag/GS-Fe3O4/Cd(2+)) can not only increase the electrocatalytic activity towards hydrogen peroxide (H2O2) but also improve the effective immobilization of antibodies because of synergistic effect presented in Au@Ag/GS-Fe3O4/Cd(2+), which greatly extended the scope of detection. Under the optimal conditions, the proposed immunosensor was used for the detection of IgG with good linear relation in the range from 5?fg/mL to 50?ng/mL with a low detection limit of 2?fg/mL (S/N?=?3). Furthermore, the proposed immunosensor showed high sensitivity, special selectivity and long-term stability, which had promising application in bioassay analysis.

| S-EPMC4754691 | biostudies-literature

Electrochemical properties of double wall carbon nanotube electrodes

Project description:Electrochemical properties of double wall carbon nanotubes (DWNT) were assessed and compared to their single wall (SWNT) counterparts. The double and single wall carbon nanotube materials were characterized by Raman spectroscopy, scanning and transmission electron microscopy and electrochemistry. The electrochemical behavior of DWNT film electrodes was characterized by using cyclic voltammetry of ferricyanide and NADH. It is shown that while both DWNT and SWNT were significantly functionalized with oxygen containing groups, double wall carbon nanotube film electrodes show a fast electron transfer and substantial decrease of overpotential of NADH when compared to the same way treated single wall carbon nanotubes.

| S-EPMC3245571 | biostudies-literature

Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite.

Project description:In the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01-1 pg mL-1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL-1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

| S-EPMC8295321 | biostudies-literature

Carbon Nanotube-Graphene Hybrid Electrodes with Enhanced Thermo-Electrochemical Cell Properties.

Project description:Carbon nanotube-Graphene (CNT-Gr) hybrids were prepared on stainless steel substrates by the electrophoretic deposition (EPD) to make the thermo-electrochemical cell (TEC) electrodes. The as-obtained TEC electrodes were investigated by the SEM, XRD, Raman spectroscopy, tensile, and surface resistance tests. These hybrid electrodes exhibited significant improved TEC performances compared to the pristine CNT electrode. In addition, these hybrid electrodes could be optimized by tuning the contents of the graphene in the hybrids, and the CNT-Gr-0.1 hybrid electrode showed the best TEC performance with the current density of 62.8 A·m-2 and the power density of 1.15 W·m-2, 30.4% higher than the CNT electrode. The enhanced TEC performance is attributed to improvements in the electrical and thermal conductivities, as well as the adhesion between the CNT-Gr hybrid and the substrate. Meanwhile, the relative conversion efficiency of the TECs can reach 1.35%. The investigation suggests that the growth of CNT-Gr hybrid electrodes by the EPD technique may offer a promising approach for practical applications of the carbon nanomaterial-based TEC electrodes.

| S-EPMC6835304 | biostudies-literature

Thermo-Electrochemical Cells Based on Carbon Nanotube Electrodes by Electrophoretic Deposition.

Project description:Drawbacks of low efficiency and high cost of the electrode materials have restricted the wide applications of the thermo-electrochemical cells (TECs). Due to high specific areas and electrical conductivities, the low cost multi-walled carbon nanotubes (MWNTs) are promising alternative electrode materials. In this work, the MWNT films of up to 16 cm2 were synthesized on stainless steel substrates by the electrophoretic deposition (EPD) to make the thermo-electrochemical electrodes. MWNT electrodes based on TECs were characterized by cyclic voltammetry and the long-term stability tests with the potassium ferri/ferrocyanide electrolyte. The TECs reached the current density of 45.2 A m-2 and the maximum power density of 0.82 W m-2. The relative power conversion efficiency of the MWNT electrode is 50 % higher than that for the Pt electrode. Meanwhile, the TECs was operated continuously for 300 h without performance degradation. With the priorities of low cost and simple fabrication, EPD-based MWNT TECs may become commercially viable.

| S-EPMC6223680 | biostudies-literature

Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.

Project description:Gold microelectrode arrays functionalized with dithiobis(succinimidyl propionate) self-assembled monolayer (SAM) have been used to fabricate an ultrasensitive, disposable, electrochemical cortisol immunosensor. Cortisol specific monoclonal antibody (C-Mab) was covalently immobilized on the surface of gold microelectrode array and the sensors were exposed to solutions with different cortisol concentration. After C-Mab binding, unreacted active groups of DTSP were blocked using ethanol amine (EA) and label-free electrochemical impedance (EIS) technique was used to determine cortisol concentration. EIS results confirmed that EA/C-Mab/DTSP/Au based biosensor can accurately detect cortisol in the range of 1pM-100nM. The biosensor was successfully used for the measurement of cortisol in interstitial fluid in vitro. This research establishes the feasibility of using impedance based biosensor architecture for disposable, wearable cortisol detector.

| S-EPMC2869627 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data