Ontology highlight
ABSTRACT:
SUBMITTER: Lennon NJ
PROVIDER: S-EPMC2872875 | biostudies-other | 2010
REPOSITORIES: biostudies-other
Genome biology 20100205 2
We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramati ...[more]