Ontology highlight
ABSTRACT:
SUBMITTER: Ye S
PROVIDER: S-EPMC2875874 | biostudies-other | 2009 Jun
REPOSITORIES: biostudies-other
Ye Shixin S Huber Thomas T Vogel Reiner R Sakmar Thomas P TP
Nature chemical biology 20090426 6
We demonstrate the site-directed incorporation of an IR-active amino acid, p-azido-L-phenylalanine (azidoF, 1), into the G protein-coupled receptor rhodopsin using amber codon suppression technology. The antisymmetric stretch vibration of the azido group absorbs at approximately 2,100 cm(-1) in a clear spectral window and is sensitive to its electrostatic environment. We used FTIR difference spectroscopy to monitor the azido probe and show that the electrostatic environments of specific interhel ...[more]