Ontology highlight
ABSTRACT:
SUBMITTER: Ryan JA
PROVIDER: S-EPMC2919900 | biostudies-other | 2010 Jul
REPOSITORIES: biostudies-other
Ryan Jeremy A JA Brunelle Joslyn K JK Letai Anthony A
Proceedings of the National Academy of Sciences of the United States of America 20100706 29
It is well established that CD4(+) CD8(+) thymocytes are more sensitive to myriad death stimuli than CD4(+) or CD8(+) single positive (SP) thymocytes. The mechanism behind this hypersensitivity to apoptosis of CD4(+) CD8(+) thymocytes is not understood. To test whether the difference lay in the apoptotic preset of mitochondria, established by the BCL-2 family of proteins, we developed a method, FACS-based BH3 profiling. Using this tool, we could discriminate thymocyte subpopulations and demonstr ...[more]