Ontology highlight
ABSTRACT:
SUBMITTER: Meyer M
PROVIDER: S-EPMC2930558 | biostudies-other | 2010 Aug
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20100804 34
Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7-4.5% of murine one-cell embryos upon the ...[more]