Ontology highlight
ABSTRACT:
SUBMITTER: Strub K
PROVIDER: S-EPMC298578 | biostudies-other | 1989 Dec
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 19891201 24
Using an enhancement of the polymerase chain reaction (PCR) technique, we have isolated a complementary DNA encoding SRP14 (14-kDa subunit), one of six proteins contained in the signal recognition particle (SRP). Several pools of degenerate oligonucleotides encoding different peptide sequences of SRP14 were used to generate amplified DNA by the PCR. A cross-hybridization procedure was developed to identify the authentic SRP14 cDNA clone among the amplified DNA products obtained by PCR. The basis ...[more]