Ontology highlight
ABSTRACT:
SUBMITTER: Sarma K
PROVIDER: S-EPMC3009817 | biostudies-other | 2010 Dec
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20101206 51
A large fraction of the mammalian genome is transcribed into long noncoding RNAs. The RNAs remain largely uncharacterized as the field awaits new technologies to aid functional analysis. Here, we describe a unique use of locked nucleic acids (LNAs) for studying nuclear long noncoding RNA, an RNA subclass that has been less amenable to traditional knockdown techniques. We target LNAs at Xist RNA and show displacement from the X chromosome with fast kinetics. Xist transcript stability is not affec ...[more]