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T-cell receptor signals direct the composition and function of the memory CD8+ T-cell pool.

ABSTRACT: SH2 domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76) nucleates a signaling complex critical for T-cell receptor (TCR) signal propagation. Mutations in the tyrosines of SLP-76 result in graded defects in TCR-induced signals depending on the tyrosine(s) affected. Here we use 2 strains of genomic knock-in mice expressing tyrosine to phenylalanine mutations to examine the role of TCR signals in the differentiation of effector and memory CD8(+) T cells in response to infection in vivo. Our data support a model in which altered TCR signals can determine the rate of memory versus effector cell differentiation independent of initial T-cell expansion. Furthermore, we show that TCR signals sufficient to promote CD8(+) T-cell differentiation are different from those required to elicit inflammatory cytokine production.

SUBMITTER: Smith-Garvin JE

PROVIDER: S-EPMC3031403 | biostudies-other | 2010 Dec

REPOSITORIES: biostudies-other

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T-cell receptor signals direct the composition and function of the memory CD8+ T-cell pool.

Smith-Garvin Jennifer E JE Burns Jeremy C JC Gohil Mercy M Zou Tao T Kim Jiyeon S JS Maltzman Jonathan S JS Wherry E John EJ Koretzky Gary A GA Jordan Martha S MS

Blood 20100916 25

SH2 domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76) nucleates a signaling complex critical for T-cell receptor (TCR) signal propagation. Mutations in the tyrosines of SLP-76 result in graded defects in TCR-induced signals depending on the tyrosine(s) affected. Here we use 2 strains of genomic knock-in mice expressing tyrosine to phenylalanine mutations to examine the role of TCR signals in the differentiation of effector and memory CD8(+) T cells in response to infection in vivo. O ...[more]

PMID: 20847203

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