Unknown

Dataset Information

0

MITF-siRNA formulation is a safe and effective therapy for human melasma.


ABSTRACT: It is unclear whether siRNA-based agents can be a safe and effective therapy for diseases. In this study, we demonstrate that microphthalmia-associated transcription factor-siRNA (MITF-siR)-silenced MITF gene expression effectively induced a significant reduction in tyrosinase (TYR), tyrosinase-related protein 1, and melanocortin 1 receptor (MC1R) levels. The siRNAs caused obvious inhibition of melanin synthesis and melanoma cell apoptosis. Using a novel type of transdermal peptide, we developed the formulation of an MITF-siR cream. Results demonstrated that hyperpigmented facial lesions of siRNA-treated subjects were significantly lighter after 12 weeks of therapy than before treatment (P < 0.001); overall improvement was first noted after 4 weeks of siRNA treatment. At the end of treatment, clinical and colorimetric evaluations demonstrated a 90.4% lightening of the siRNA-treated lesions toward normal skin color. The relative melanin contents in the lesions and adjacent normal skin were decreased by 26% and 7.4%, respectively, after treatment with the MITF-siR formulation. Topical application of siRNA formulation significantly lightens brown facial hypermelanosis and lightens normal skin in Asian individuals. This treatment represents a safe and effective therapy for melasma, suggesting that siRNA-based agents could be developed for treating other diseases such as melanoma.

SUBMITTER: Yi X 

PROVIDER: S-EPMC3034856 | biostudies-other | 2011 Feb

REPOSITORIES: biostudies-other

Similar Datasets

2024-01-16 | GSE241939 | GEO
| S-EPMC4452141 | biostudies-literature
| S-EPMC3125526 | biostudies-literature
| S-EPMC7077636 | biostudies-literature
| S-EPMC5415343 | biostudies-literature
| S-EPMC3734668 | biostudies-literature
| S-EPMC8407678 | biostudies-literature
| S-EPMC5418099 | biostudies-literature
| S-EPMC7116733 | biostudies-literature
| S-EPMC5811182 | biostudies-literature