Dataset Information

Effects of 15d-PGJ?-loaded poly(D,L-lactide-co-glycolide) nanocapsules on inflammation.

ABSTRACT: BACKGROUND AND PURPOSE: The PPAR-? agonist 15d-PGJ? is a potent anti-inflammatory agent but only at high doses. To improve the efficiency of 15d-PGJ?, we used poly(D,L-lactide-co-glycolide) nanocapsules to encapsulate it, and function as a drug carrier system. The effects of these loaded nanocapsules (15d-PGJ?-NC) on inflammation induced by different stimuli were compared with those of free 15d-PGJ?. EXPERIMENTAL APPROACH: Mice were pretreated (s.c.) with either 15d-PGJ?-NC or unloaded 15d-PGJ? (3, 10 or 30 µg·kg?¹), before induction of an inflammatory response by i.p. injection of either endotoxin (LPS), carrageenan (Cg) or mBSA (immune response). KEY RESULTS: The 15d-PGJ?-NC complex did not display changes in physico-chemical parameters or drug association efficiency over time, and was stable for up to 60 days of storage. Neutrophil migration induced by i.p. administration of LPS, Cg or mBSA was inhibited by 15d-PGJ?-NC, but not by unloaded 15d-PGJ?. In the Cg model, 15d-PGJ?-NC markedly inhibited serum levels of the pro-inflammatory cytokines TNF-?, IL-1? and IL-12p70. Importantly, 15d-PGJ?-NC released high amounts of 15d-PGJ?, reaching a peak between 2 and 8 h after administration. 15d-PGJ ? was detected in mouse serum after 24 h, indicating sustained release from the carrier. When the same concentration of unloaded 15d-PGJ? was administered, only small amounts of 15d-PGJ? were found in the serum after a few hours. CONCLUSIONS AND IMPLICATIONS: The present findings clearly indicate the potential of the novel anti-inflammatory 15d-PGJ? carrier formulation, administered systemically. The formulation enables the use of a much smaller drug dose, and is significantly more effective compared with unloaded 15d-PGJ?.

SUBMITTER: Alves C

PROVIDER: S-EPMC3041252 | biostudies-other | 2011 Feb

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

Effects of 15d-PGJ₂-loaded poly(D,L-lactide-co-glycolide) nanocapsules on inflammation.

Alves Cf C de Melo Nfs N Fraceto Lf L de Araújo Dr D Napimoga Mh M

British journal of pharmacology 20110201 3

<h4>Background and purpose</h4>The PPAR-γ agonist 15d-PGJ₂ is a potent anti-inflammatory agent but only at high doses. To improve the efficiency of 15d-PGJ₂, we used poly(D,L-lactide-co-glycolide) nanocapsules to encapsulate it, and function as a drug carrier system. The effects of these loaded nanocapsules (15d-PGJ₂-NC) on inflammation induced by different stimuli were compared with those of free 15d-PGJ₂.<h4>Experimental approach</h4>Mice were pretreated (s.c.) with either 15d-PGJ₂-NC or unloa ...[more]

PMID: 20883476

Similar Datasets

Project description:Fluorescent biomaterials have attracted significant research efforts in the past decades. Herein, we report a new series of biodegradable, fluorescence imaging-enabled copolymers, biodegradable photoluminescent poly(lactide-co-glycolide) (BPLP-co-PLGA). Photoluminescence characterization shows that BPLP-co-PLGA solutions, films and nanoparticles all exhibit strong, tunable and stable photoluminescence. By adjusting the molar ratios of L-lactide (LA)/glycolide (GA) and (LA+GA)/BPLP, full degradation of BPLP-co-PLGA can be achieved in 8-16 weeks. The fluorescence decay behavior of BPLP-co-PLGA can be used for non-invasive monitoring of material degradation. In vitro cytotoxicity and in vivo foreign body response evaluations demonstrate that BPLP-co-PLGA exhibits similar biocompatibility to poly(lactide-co-glycolide) (PLGA). The imaging-enabled BPLP-co-PLGA was fabricated into porous scaffolds whose degradation can be monitored through non-invasive imaging and nanoparticles that show theranostic potential demonstrated by fluorescent cellular labeling, imaging and sustained 5-fluorouracil delivery. The development of inherently fluorescent PLGA copolymers is expected to impact the use of already widely accepted PLGA polymers for applications where fluorescent properties are highly desired but limited by the conventional use of cytotoxic quantum dots and photobleaching organic dyes.Statement of significanceThis manuscript describes a novel strategy of conferring intrinsic photoluminescence to the widely used biodegradable polymers, poly(lactide-co-glycolide) without introducing any cytotoxic quantum dots or photo-bleaching organic dyes, which may greatly expand the applications of these polymers in where fluorescent properties are highly desired. Given the already significant impact generated by the use of PLGA and alike, this work contributes to fluorescence chemistry and new functional biomaterial design and will potentially generate significant impact on many fields of applications such as tissue engineering, molecular imaging and labeling, and drug delivery.

Dataset Information

Effects of 15d-PGJ?-loaded poly(D,L-lactide-co-glycolide) nanocapsules on inflammation.

Publications

Effects of 15d-PGJ₂-loaded poly(D,L-lactide-co-glycolide) nanocapsules on inflammation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets