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Transgenic overexpression of PKC? in the mouse prostate induces preneoplastic lesions.


ABSTRACT: It is well established that protein kinase C (PKC) isozymes play distinctive roles in mitogenic and survival signaling as well as in cancer progression. PKC?, the product of the PRKCE gene, is up-regulated in various types of cancers including prostate, lung and breast cancer. To address a potential role for PKCs in prostate cancer progression we generated three mouse transgenic lines expressing PKC?, PKC?, or PKC? in the prostate epithelium under the control of the rat probasin (PB) promoter. Whereas PB-PKC? and PB-PKC? mice did not show any evident phenotype, PB-PKC? mice developed prostate hyperplasia as well as prostate intraepithelial neoplasia (PIN) that displayed enhanced phospho-Akt, phospho-S6, and phospho-Stat3 levels, as well as enhanced resistance to apoptotic stimuli. PKC? overexpression was insufficient to drive neoplastic changes in the mouse prostate. Notably, overexpression of PKC? by adenoviral means in normal immortalized RWPE-1 prostate cells confers a growth advantage and hyperactivation of Erk and Akt. Our results argue for a causal link between PKC? overexpression and prostate cancer development.

SUBMITTER: Benavides F 

PROVIDER: S-EPMC3048798 | biostudies-other | 2011 Jan

REPOSITORIES: biostudies-other

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Transgenic overexpression of PKCε in the mouse prostate induces preneoplastic lesions.

Benavides Fernando F   Blando Jorge J   Perez Carlos J CJ   Garg Rachana R   Conti Claudio J CJ   DiGiovanni John J   Kazanietz Marcelo G MG  

Cell cycle (Georgetown, Tex.) 20110115 2


It is well established that protein kinase C (PKC) isozymes play distinctive roles in mitogenic and survival signaling as well as in cancer progression. PKCε, the product of the PRKCE gene, is up-regulated in various types of cancers including prostate, lung and breast cancer. To address a potential role for PKCs in prostate cancer progression we generated three mouse transgenic lines expressing PKCα, PKCδ, or PKCε in the prostate epithelium under the control of the rat probasin (PB) promoter. W  ...[more]

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