Ontology highlight
ABSTRACT:
SUBMITTER: Ivanova NB
PROVIDER: S-EPMC307135 | biostudies-other | 1995 Aug
REPOSITORIES: biostudies-other
Nucleic acids research 19950801 15
A novel method for identification of differentially expressed genes has been developed. It is based on the consecutive restriction digestions of 3' terminal cDNA fragments to produce a fingerprint of gene expression. cDNA molecules are synthesized using a biotinylated oligo(dT) primer, digested with a frequently cutting restriction endonuclease and the 3'-terminal restriction fragments are isolated using streptavidin microbeads. After amplification by PCR, cDNA fragments are immobilized again on ...[more]