Ontology highlight
ABSTRACT:
SUBMITTER: Meijer WJ
PROVIDER: S-EPMC307180 | biostudies-other | 1995 Aug
REPOSITORIES: biostudies-other
Meijer W J WJ de Boer A J AJ van Tongeren S S Venema G G Bron S S
Nucleic acids research 19950801 16
A 3.1 kb fragment of the large (approximately 55 kb) Bacillus subtilis plasmid pLS20 containing all the information for autonomous replication was cloned and sequenced. In contrast to the parental plasmid, derived minireplicons were unstably maintained. Using deletion analysis the fragment essential and sufficient for replication was delineated to 1.1 kb. This 1.1 kb fragment is located between two divergently transcribed genes, denoted orfA and orfB, neither of which is required for replication ...[more]