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The DNA (cytosine-5) methyltransferases.

ABSTRACT: The m5C-MTases form a closely-knit family of enzymes in which common amino acid sequence motifs almost certainly translate into common structural and functional elements. These common elements are located predominantly in a single structural domain that performs the chemistry of the reaction. Sequence-specific DNA recognition is accomplished by a separate domain that contains recognition elements not seen in other structures. This, combined with the novel and unexpected mechanistic feature of trapping a base out of the DNA helix, makes the m5C-MTases an intriguing class of enzymes for further study. The reaction pathway has suddenly become more complicated because of the base-flipping and much remains to be learned about the DNA recognition elements in the family members for which structural information is not yet available.

SUBMITTER: Kumar S

PROVIDER: S-EPMC307737 | biostudies-other | 1994 Jan

REPOSITORIES: biostudies-other

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The DNA (cytosine-5) methyltransferases.

Kumar S S Cheng X X Klimasauskas S S Mi S S Posfai J J Roberts R J RJ Wilson G G GG

Nucleic acids research 19940101 1

The m5C-MTases form a closely-knit family of enzymes in which common amino acid sequence motifs almost certainly translate into common structural and functional elements. These common elements are located predominantly in a single structural domain that performs the chemistry of the reaction. Sequence-specific DNA recognition is accomplished by a separate domain that contains recognition elements not seen in other structures. This, combined with the novel and unexpected mechanistic feature of tr ...[more]

PMID: 8127644

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