Ontology highlight
ABSTRACT:
SUBMITTER: Holt N
PROVIDER: S-EPMC3080757 | biostudies-other | 2010 Aug
REPOSITORIES: biostudies-other
Holt Nathalia N Wang Jianbin J Kim Kenneth K Friedman Geoffrey G Wang Xingchao X Taupin Vanessa V Crooks Gay M GM Kohn Donald B DB Gregory Philip D PD Holmes Michael C MC Cannon Paula M PM
Nature biotechnology 20100702 8
CCR5 is the major HIV-1 co-receptor, and individuals homozygous for a 32-bp deletion in CCR5 are resistant to infection by CCR5-tropic HIV-1. Using engineered zinc-finger nucleases (ZFNs), we disrupted CCR5 in human CD34(+) hematopoietic stem/progenitor cells (HSPCs) at a mean frequency of 17% of the total alleles in a population. This procedure produces both mono- and bi-allelically disrupted cells. ZFN-treated HSPCs retained the ability to engraft NOD/SCID/IL2rgamma(null) mice and gave rise to ...[more]