Ontology highlight
ABSTRACT:
SUBMITTER: Weigand JE
PROVIDER: S-EPMC3082870 | biostudies-other | 2011 Apr
REPOSITORIES: biostudies-other
Weigand Julia E JE Schmidtke Sina R SR Will Tristan J TJ Duchardt-Ferner Elke E Hammann Christian C Wöhnert Jens J Suess Beatrix B
Nucleic acids research 20101211 8
While many different RNA aptamers have been identified that bind to a plethora of small molecules only very few are capable of acting as engineered riboswitches. Even for aptamers binding the same ligand large differences in their regulatory potential were observed. We address here the molecular basis for these differences by using a set of unrelated neomycin-binding aptamers. UV melting analyses showed that regulating aptamers are thermally stabilized to a significantly higher degree upon ligan ...[more]