Ontology highlight
ABSTRACT:
SUBMITTER: Dolnick BJ
PROVIDER: S-EPMC309410 | biostudies-other | 1993 Apr
REPOSITORIES: biostudies-other
Nucleic acids research 19930401 8
Based upon reverse transcription and polymerase chain reaction results with human KB cell RNA, a cDNA (i.e., 3'rTS1, 1557 nt) with complementarity to thymidylate synthase mRNA was cloned and sequenced. Northern blot analysis showed that 3'rTS1 corresponded to a cytoplasmic 1.8 kb RNA found in several tumor cell lines. The remaining 5'region of this antisense RNA was cloned by a RACE (Rapid Amplification of cDNA Ends) procedure. A full length cDNA (i.e., rTS, 1811 nt) was generated by splicing 3' ...[more]