Ontology highlight
ABSTRACT:
SUBMITTER: Van den Ouweland AM
PROVIDER: S-EPMC317862 | biostudies-other | 1989 May
REPOSITORIES: biostudies-other
Van den Ouweland A M AM Van Duijnhoven H L HL Deichmann K A KA Van Groningen J J JJ de Leij L L Van de Ven W J WJ
Nucleic acids research 19890501 10
The monoclonal antibody MOC-32 detected a 40 kDa protein in Western blot analysis. Immunological screening of an expression library of human SCLC cells with MOC-32 led to the isolation of overlapping cDNA clones. One of these, cHD4, was 1.0 kbp long and of about the same size as its corresponding mRNA. Preceded by an in phase stop codon, an open reading frame of 885 bp was present in cHD4 and a translational product of only 33 kDa could be calculated. Biochemical and immunological analysis estab ...[more]