Determination of binding kinetics using whispering gallery mode microcavities.
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ABSTRACT: Silica optical microcavity sensors show great promise in the kinetic evaluation of binding pairs, fundamental in understanding biomolecular interactions. Here, we develop and demonstrate a novel platform, based on bioconjugated silica microsphere resonators, to study the binding kinetics of the biotin-streptavidin system. We characterize the optical performance, verify the covalent attachment of biotin to the surface, and perform streptavidin detection experiments. We perform preliminary kinetic analysis of the detection data which shows the potential of whispering gallery mode resonators in the determination of the dissociation constant of the binding pair, which is in good agreement with previously published values.
SUBMITTER: Soteropulos CE
PROVIDER: S-EPMC3189252 | biostudies-other | 2011 Sep
REPOSITORIES: biostudies-other
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