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Activation of junB by PKC and PKA signal transduction through a novel cis-acting element.


ABSTRACT: The product of the junB gene, a gene homologous to the proto-oncogene c-jun, is a component of transcription factor AP-1. JunB expression is modulated by a wide variety of extracellular stimuli, such as serum, growth factors, phorbol esters (TPA) and activators of protein kinase A (PKA). In order to study the molecular basis of this complex regulation, we have cloned the mouse junB gene from a genomic testis library, and characterized the junB promoter. Here we show that the junB promoter is activated by serum, TPA, and activated PKA. Sequences located between -91 and -44 are necessary for induction. These sequences contain a CAAT box, a G-C rich region and a previously undescribed inverted repeat (IR). The IR element can mediate induction by TPA and PKA when coupled to a heterologous promoter, and specifically binds a protein of 110 kD.

SUBMITTER: de Groot RP 

PROVIDER: S-EPMC333710 | biostudies-other | 1991 Feb

REPOSITORIES: biostudies-other

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Activation of junB by PKC and PKA signal transduction through a novel cis-acting element.

de Groot R P RP   Auwerx J J   Karperien M M   Staels B B   Kruijer W W  

Nucleic acids research 19910201 4


The product of the junB gene, a gene homologous to the proto-oncogene c-jun, is a component of transcription factor AP-1. JunB expression is modulated by a wide variety of extracellular stimuli, such as serum, growth factors, phorbol esters (TPA) and activators of protein kinase A (PKA). In order to study the molecular basis of this complex regulation, we have cloned the mouse junB gene from a genomic testis library, and characterized the junB promoter. Here we show that the junB promoter is act  ...[more]

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