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Disulfide bonds are required for Serratia marcescens nuclease activity.

ABSTRACT: The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

SUBMITTER: Ball TK

PROVIDER: S-EPMC334271 | biostudies-other | 1992 Oct

REPOSITORIES: biostudies-other

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Disulfide bonds are required for Serratia marcescens nuclease activity.

Ball T K TK Suh Y Y Benedik M J MJ

Nucleic acids research 19921001 19

The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm. ...[more]

PMID: 1329033

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