Dataset Information

The nucleolar channel system reliably marks the midluteal endometrium regardless of fertility status: a fresh look at an old organelle.

ABSTRACT: To determine whether nucleolar channel systems (NCSs) in the midluteal endometrium are associated with overall fertility status and/or with unexplained infertility.Retrospective and prospective clinical studies.Repository of stored specimens from prior multicenter study and private infertility center.Retrospective study that included 97 women (49 fertile couples, 48 infertile couples) who had been randomized for endometrial biopsy during the midluteal or late luteal phase. The prospective study included 78 women with a variety of infertility diagnoses.Endometrial biopsies were obtained and assessed for the presence of NCSs by indirect immunofluorescence.The presence of NCS was graded semiquantitatively and dichotomized as normal versus low or absent.Normal presence of NCS was significantly associated with the midluteal phase compared with the late luteal phase (80% vs. 29%). However, there was no association between presence of NCS and fertility status or between presence of NCS and unexplained infertility.Midluteal phase endometrium consistently forms NCSs regardless of fertility status, including unexplained infertility. This indicates a possible role for the NCS in initiating the window of endometrial receptivity. However, the consistent presence of NCSs across several different types of infertility challenges the likelihood that inadequate secretory transformation is a cause of infertility.

SUBMITTER: Rybak EA

PROVIDER: S-EPMC3347775 | biostudies-other | 2011 Mar

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

The nucleolar channel system reliably marks the midluteal endometrium regardless of fertility status: a fresh look at an old organelle.

Rybak Eli A EA Szmyga Michael J MJ Zapantis Gregory G Rausch Mary M Beshay Victor E VE Polotsky Alex J AJ Coutifaris Christos C Carr Bruce R BR Santoro Nanette N Meier U Thomas UT

Fertility and sterility 20101110 4

<h4>Objective</h4>To determine whether nucleolar channel systems (NCSs) in the midluteal endometrium are associated with overall fertility status and/or with unexplained infertility.<h4>Design</h4>Retrospective and prospective clinical studies.<h4>Setting</h4>Repository of stored specimens from prior multicenter study and private infertility center.<h4>Patient(s)</h4>Retrospective study that included 97 women (49 fertile couples, 48 infertile couples) who had been randomized for endometrial biop ...[more]

PMID: 21067716

Similar Datasets

Project description:Mechanical stress from haemodynamic perturbations or interventional manipulation of epicardial coronary atherosclerotic plaques with inflammatory destabilization can release particulate debris, thrombotic material and soluble substances into the coronary circulation. The physical material obstructs the coronary microcirculation, whereas the soluble substances induce endothelial dysfunction and facilitate vasoconstriction. Coronary microvascular obstruction and dysfunction result in patchy microinfarcts accompanied by an inflammatory reaction, both of which contribute to progressive myocardial contractile dysfunction. In clinical studies, the benefit of protection devices to retrieve atherothrombotic debris during percutaneous coronary interventions has been modest, and the treatment of microembolization has mostly relied on antiplatelet and vasodilator agents. The past 25 years have witnessed a relative proportional increase in non-ST-segment elevation myocardial infarction in the presentation of acute coronary syndromes. An associated increase in the incidence of plaque erosion rather than rupture has also been recognized as a key mechanism in the past decade. We propose that coronary microembolization is a decisive link between plaque erosion at the culprit lesion and the manifestation of non-ST-segment elevation myocardial infarction. In this Review, we characterize the features and mechanisms of coronary microembolization and discuss the clinical trials of drugs and devices for prevention and treatment. In this Review, Kleinbongard and Heusch characterize the features and mechanisms of coronary microembolization and discuss the clinical trials of drugs and devices for the prevention and treatment of this phenomenon. Key points Coronary microembolization results from spontaneous or interventional erosion or rupture of an epicardial coronary atherosclerotic plaque. Atherothrombotic plaque debris causes physical obstruction of coronary microvessels and results in microinfarcts with an inflammatory response. The surviving myocardium around the patchy microinfarcts has reduced contractile function as a result of inflammatory signal transduction leading to myofibrillar oxidation. Atherosclerotic plaque erosion or rupture releases soluble substances that induce coronary microvascular endothelial dysfunction, vasoconstriction, platelet aggregation and coagulation. Routine use of interventional protection devices does not improve patient outcomes but these devices are indicated in cases of high atherothrombotic plaque burden. Plaque stabilization with statins and canakinumab prevents coronary microembolization, whereas platelet inhibitors, vasodilators and interleukin antagonists attenuate coronary microvascular impairment.

Dataset Information

The nucleolar channel system reliably marks the midluteal endometrium regardless of fertility status: a fresh look at an old organelle.

Publications

The nucleolar channel system reliably marks the midluteal endometrium regardless of fertility status: a fresh look at an old organelle.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets