Unknown

Dataset Information

0

Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster.


ABSTRACT: We present the complete nucleotide and deduced amino acid sequence for the gene encoding Drosophila sn-glycerol-3-phosphate dehydrogenase. A transcription unit of 5kb was identified which is composed of eight protein encoding exons. Three classes of transcripts were shown to differ only in the 3'-end and to code for three protein isoforms each with a different C-terminal amino acid sequence. Each transcript is shown to arise through the differential expression of three isotype-specific exons at the 3'-end of the gene by a developmentally regulated process of 3'-end formation and alternate splicing pathways of the pre-mRNA. In contrast, the 5'-end of the gene is simple in structure and each mRNA is transcribed from the same promoter sequence. A comparison of the organization of the Drosophila and murine genes and the primary amino acid sequence between a total of four species indicates that the GPDH gene-enzyme system is highly conserved and is evolving slowly.

SUBMITTER: Bewley GC 

PROVIDER: S-EPMC335027 | biostudies-other | 1989 Nov

REPOSITORIES: biostudies-other

altmetric image

Publications

Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster.

Bewley G C GC   Cook J L JL   Kusakabe S S   Mukai T T   Rigby D L DL   Chambers G K GK  

Nucleic acids research 19891101 21


We present the complete nucleotide and deduced amino acid sequence for the gene encoding Drosophila sn-glycerol-3-phosphate dehydrogenase. A transcription unit of 5kb was identified which is composed of eight protein encoding exons. Three classes of transcripts were shown to differ only in the 3'-end and to code for three protein isoforms each with a different C-terminal amino acid sequence. Each transcript is shown to arise through the differential expression of three isotype-specific exons at  ...[more]

Similar Datasets

| S-EPMC4571891 | biostudies-literature
| S-EPMC40297 | biostudies-other
| S-EPMC3933693 | biostudies-literature
| S-EPMC6765128 | biostudies-literature
| S-EPMC297548 | biostudies-other
| S-EPMC205338 | biostudies-other
| S-EPMC207162 | biostudies-other
| S-EPMC2583299 | biostudies-literature
| S-EPMC6613444 | biostudies-literature
| S-EPMC3515364 | biostudies-literature