Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation.

ABSTRACT: We experimentally investigate the effects of high electric field on living cells inside a charged droplet under electrophoretic actuation. When an aqueous droplet suspended in a dielectric liquid contacts with electrified electrode, the droplet acquires charge. This charged droplet undergoes electrophoretic motion under strong electric field (1-3 kV/cm), which can be used as a droplet manipulation method in biomicrofluidic applications. However, because strong electric field and use of dielectric oil can be a harmful environment for living cells, the biological feasibilities have been tested. Trypan blue test and cell growth test have been performed to check the viability and proliferation of cells in a droplet under various electric field strengths and actuation times. We have not observed any noticeable influence of electric field and silicone oil on the viability and proliferation of cells, which indicates that electrophoresis could be safely used as a manipulation method for a droplet containing living biological system.

SUBMITTER: Im DJ

PROVIDER: S-EPMC3364810 | biostudies-other | 2011 Dec

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field.

Project description:We have developed a gene transfection method called water-in-oil droplet electroporation (EP) that uses a dielectric oil and a liquid droplet containing live cells and exogenous DNA. When a cell suspension droplet is placed between a pair of electrodes, an intense DC electric field can induce droplet deformation, resulting in an instantaneous short circuit caused by the droplet elongating and contacting the two electrodes simultaneously. Small transient pores are generated in the cell membrane during the short, allowing the introduction of exogenous DNA into the cells. The droplet EP was characterized by varying the following experimental parameters: applied voltage, number of short circuits, type of medium (electric conductivity), concentration of exogenous DNA, and size of the droplet. In addition, the formation of transient pores in the cell membrane during droplet EP and the transfection efficiency were evaluated.

| S-EPMC5613697 | biostudies-other

Multipolar spatial electric field modulation for freeform electroactive hydrogel actuation.

Project description:Electroactive hydrogels that exhibit large deformation in response to an electric field have received significant attention as a potential actuating material for soft actuators and artificial muscle. However, their mechanical actuation has been limited in simple bending or folding due to uniform electric field modulation. To implement complex movements, a pre-program, such as a hinge and a multilayer pattern, is usually required for the actuator in advance. Here, we propose a reprogrammable actuating method and sophisticated manipulation by using multipolar three-dimensional electric field modulation without pre-program. Through the multipolar spatial electric field modulator, which controls the polarity/intensity of the electric field in three-dimensions, complex three-dimensional (3D) actuation of single hydrogels are achieved. Also, air bubbles generated during operation in the conventional horizontal configuration are not an issue in the proposed new vertical configuration. We demonstrate soft robotic actuators, including basic bending mechanics in terms of controllability and reliability, and several 3D shapes having positive and negative curvature can easily be achieved in a single sheet, paving the way for continuously reconfigurable materials.

| S-EPMC7015902 | biostudies-literature

Rotating Motion of an Oil Droplet in a Circular Channel Subjected to a Transverse Alternating Electric Field.

Project description:The transient behavior of a silicone oil droplet passing through a circular channel in which castor oil passes as a surrounding fluid is investigated in both a periodically intermittent alternating electric field and a sinusoidal alternating electric field. When a periodically intermittent electric field with a low frequency and medium strength is applied, the droplet first undergoes ellipsoidal deformation where the major axis is oriented in the direction between the electric field direction and the direction normal to the electric field and electrorotation occurs. The inclined major axis tilts away from the electric field direction and then approaches it. For the case where a sinusoidal alternating electric field with medium strength is applied, electrorotation occurs when the frequency of the alternating sinusoidal electric field is low, whereas the droplet periodically deforms between a prolate and an oblate ellipsoid at certain frequencies. When the frequency of the electric field applied is high, the droplet remains spherical or undergoes deformation to a quasi-steady prolate ellipsoid with an aspect ratio of almost unity.

| S-EPMC6641384 | biostudies-literature

Electric field assisted motion of a mercury droplet.

Project description:Field-assisted self-assembly, motion, and manipulation of droplets have gained much attention in the past decades. We exhibit an electric field manipulation of the motion of a liquid metal (mercury) droplet submerged in a conductive liquid medium (a solution of sulfuric acid). A mercury droplet moves toward the cathode and its path selection is always given by the steepest descent of the local electric field potential. Utilizing this unique behavior, we present several examples of droplet motions, including maze solving, electro-levitation, and motion on a diverted path between parallel electrodes by controlling the conductivity of the medium. We also present an experimental demonstration of Fermat's principle in a non-optical system, namely a mercury droplet moving along a refracted path between electrodes in a domain having two different conductivities.

| S-EPMC7854757 | biostudies-literature

Electric Field-Driven Liquid Metal Droplet Generation and Direction Manipulation.

Project description:A gallium-based liquid metal got high attention recently, due to the excellent material properties that are useful in various research areas. We report here on electric field-induced liquid metal droplet generation and falling direction manipulation. The well-analyzed electro-hydrodynamic method is a selectable way to control the liquid metal, as the liquid metal is conductive. The electric field-induced liquid metal manipulation can be affected by the flow rate (0.05~0.2 mL/min), voltage (0~7 kV), and distance (15 and 30 mm) between electrodes, which changes the volume of the electric field-induced generated liquid metal droplet and the number of the generated droplets. When the electric field intensity increases or the flow rate increases, the generated droplet volume decreases, and the number of droplets increases. With the highest voltage of 7 kV with 15 mm between electrodes at the 0.2 mL/min flow rate, the lowest volume and the largest number of the generated droplets for 10 s were ~10 nL and 541, respectively. Additionally, we controlled the direction of the generated droplet by changing the electric field. The direction of the liquid metal droplet was controlled with the maximum angle of ~12°. Moreover, we exhibited a short circuit demonstration by controlling the volume or falling direction of the generated liquid metal droplet with an applied electric field.

| S-EPMC8471384 | biostudies-literature

Electropermanent magnet actuation for droplet ferromicrofluidics.

Project description:Droplet actuation is an essential mechanism for droplet-based microfluidic systems. On-demand electromagnetic actuation is used in a ferrofluid-based microfluidic system for water droplet displacement. Electropermanent magnets (EPMs) are used to induce 50 mT magnetic fields in a ferrofluid filled microchannel with gradients up to 6.4 × 104 kA/m2. Short 50 µs current pulses activate the electropermanent magnets and generate negative magnetophoretic forces that range from 10 to 70 nN on 40 to 80 µm water-in-ferrofluid droplets. Maximum droplet displacement velocities of up to 300 µm/s are obtained under flow and no-flow conditions. Electropermanent magnet-activated droplet sorting under continuous flow is demonstrated using a split-junction microfluidic design.

| S-EPMC5003119 | biostudies-literature

Phase Transitions of Oppositely Charged Colloidal Particles Driven by Alternating Current Electric Field.

Project description:We study systems containing oppositely charged colloidal particles under applied alternating current electric fields (AC fields) using overdamped Langevin dynamics simulations in three dimensions. We obtain jammed bands perpendicular to the field direction under intermediate frequencies and lanes parallel with the field under low frequencies. These structures also depend upon the particle charges. The pathway for generating jammed bands follows a stepwise mechanism, and intermediate bands are observed during lane formation in some systems. We investigate the component of the pressure tensors in the direction parallel to the field and observe that the jammed to lane transition occurs at a critical value for this pressure. We also find that the stable steady states appear to satisfy the principle of maximum entropy production. Our results may help to improve the understand of the underlying mechanisms for these types of dynamic phase transitions and the subsequent cooperative assemblies of colloidal particles under such non-equilibrium conditions.

| S-EPMC8023798 | biostudies-literature

Citrate-capped gold nanoparticle electrophoretic heat production in response to a time-varying radiofrequency electric-field.

Project description:The evaluation of heat production from gold nanoparticles (AuNPs) irradiated with radiofrequency (RF) energy has been problematic due to Joule heating of their background ionic buffer suspensions. Insights into the physical heating mechanism of nanomaterials under RF excitations must be obtained if they are to have applications in fields such as nanoparticle-targeted hyperthermia for cancer therapy. By developing a purification protocol which allows for highly-stable and concentrated solutions of citrate-capped AuNPs to be suspended in high-resistivity water, we show herein, for the first time, that heat production is only evident for AuNPs of diameters ? 10 nm, indicating a unique size-dependent heating behavior not previously observed. Heat production has also shown to be linearly dependent on both AuNP concentration and total surface area, and severely attenuated upon AuNP aggregation. These relationships have been further validated using permittivity analysis across a frequency range of 10 MHz to 3 GHz, as well as static conductivity measurements. Theoretical evaluations suggest that the heating mechanism can be modeled by the electrophoretic oscillation of charged AuNPs across finite length scales in response to a time-varying electric field. It is anticipated these results will assist future development of nanoparticle-assisted heat production by RF fields for applications such as targeted cancer hyperthermia.

| S-EPMC3686525 | biostudies-literature

The influences of electric field intensity and driving force on the slip behaviour of water flow in a nanochannel.

Project description:In the present work, non-equilibrium molecular dynamics (MD) simulations are used to investigate the flow of liquid water between two metallic solid atomistic smooth walls. The present work focuses on the combined effect of external electric field and driving force on the slip behaviour and structure of liquid water at the solid-water interface. The upper wall of the set model is positively charged, and the lower wall of the model is negatively charged. The simulation results show that as the driving force increases, the slip length also increases. At a given driving force, no matter how the electric field intensity changes, there is almost no change in the slip length, so the slip length is independent of the electric field strength. In addition, the results found that there is a linear relationship between the slip length and the normalised main peak of the static structure factor under different driving forces.

| S-EPMC8457446 | biostudies-literature

Electric field-driven water dipoles: nanoscale architecture of electroporation.

Project description:Electroporation is the formation of permeabilizing structures in the cell membrane under the influence of an externally imposed electric field. The resulting increased permeability of the membrane enables a wide range of biological applications, including the delivery of normally excluded substances into cells. While electroporation is used extensively in biology, biotechnology, and medicine, its molecular mechanism is not well understood. This lack of knowledge limits the ability to control and fine-tune the process. In this article we propose a novel molecular mechanism for the electroporation of a lipid bilayer based on energetics analysis. Using molecular dynamics simulations we demonstrate that pore formation is driven by the reorganization of the interfacial water molecules. Our energetics analysis and comparisons of simulations with and without the lipid bilayer show that the process of poration is driven by field-induced reorganization of water dipoles at the water-lipid or water-vacuum interfaces into more energetically favorable configurations, with their molecular dipoles oriented in the external field. Although the contributing role of water in electroporation has been noted previously, here we propose that interfacial water molecules are the main players in the process, its initiators and drivers. The role of the lipid layer, to a first-order approximation, is then reduced to a relatively passive barrier. This new view of electroporation simplifies the study of the problem, and opens up new opportunities in both theoretical modeling of the process and experimental research to better control or to use it in new, innovative ways.

| S-EPMC3623848 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data